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KMID : 0364820140500030249
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Korean Journal of Microbiology
2014 Volume.50 No. 3 p.249 ~ p.253
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Effects of spThoc7 Deletion on Growth and mRNA Export in Fission Yeast
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Koh Eun-Jin
Yoon Jin-Ho
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Abstract
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THOC7/Mft1 is a member of the THO complex that is an evolutionally conserved assembly connecting transcription
elongation with mRNP packaging and mRNA export. In fission yeast Schizosaccharomyces pombe, an ortholog
(spThoc7) of THOC7/Mft1 was isolated by partial complementation of the lethality in synthetic lethal mutant,
SLRsm1. A deletion mutant in a diploid strain was constructed by replacing one of spThoc7-coding region with an
ura4+ gene using one-step gene disruption method. Tetrad analysis showed that the spthoc7 is nonessential for
growth. But the Δthoc7 null mutant showed slight defects of both growth and mRNA export. And the functional
spThoc7-GFP protein is localized mainly in the nucleus. These results suggest that spThoc7 is also involved in
mRNA export from the nucleus to cytoplasm.
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KEYWORD
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S. pombe, mRNA export, THO complex, Thoc7
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